Active Recombinant Human DAO Protein, His-tagged

• Cat.No.: DAO-392H
• Product Overview: Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT
• Bio-activity: Specific activity is >3.5 units/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.
• Molecular Mass: 41.6 kDa (367aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DAO D-amino-acid oxidase [ Homo sapiens ]
• Official Symbol: DAO
• Synonyms: DAO; D-amino-acid oxidase; DAMOX; D-amino acid oxidase; DAAO; OXDA; MGC35381
• Gene ID: 1610
• mRNA Refseq: NM_001917
• Protein Refseq: NP_001908
• MIM: 124050
• UniProt ID: P14920
• Chromosome Location: 12
• Pathway: Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; D-Arginine and D-ornithine metabolism, organism-specific biosystem; D-Arginine and D-ornithine metabolism, conserved biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, conserved biosystem; Glyoxylate metabolism, organism-specific biosystem
• Function: D-amino-acid oxidase activity; D-amino-acid oxidase activity; FAD binding; nucleotide binding; oxidoreductase activity; protein binding; protein dimerization activity