Recombinant Human DAP3 Protein, GST-tagged

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Recombinant Human DAP3 Protein, GST-tagged

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Cat.No. : DAP3-2333H
Product Overview : Human DAP3 full-length ORF ( NP_004623, 1 a.a. - 398 a.a.) recombinant protein with GST-tag at N-terminal.
Description : Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 69.41 kDa
AA Sequence : MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVMQ VKTFSEACLM VRKPALELLH YLKNTSFAYP AIRYLLYGEK GTGKTLSLCH VIHFCAKQDW LILHIPDAHL WVKNCRDLLQ SSYNKQRFDQ PLEASTWLKN FKTTNERFLN QIKVQEKYVW NKRESTEKGS PLGEVVEQGI TRVRNATDAV GIVLKELKRQ SSLGMFHLLV AVDGINALWG RTTLKREDKS PIAPEELALV HNLRKMMKND WHGGAIVSAL SQTGSLFKPR KAYLPQELLG KEGFDALDPF IPILVSNYNP KEFESCIQYY LENNWLQHEK APTEEGKKEL LFLSNANPSL LERHCAYL
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing: :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : DAP3 death associated protein 3 [ Homo sapiens ]
Official Symbol : DAP3
Synonyms : DAP3; death associated protein 3; 28S ribosomal protein S29, mitochondrial; bMRP 10; DAP 3; DKFZp686G12159; MGC126058; MGC126059; mitochondrial 28S ribosomal protein S29; MRP S29; MRPS29; S29mt; death-associated protein 3; ionizing radiation resistance conferring protein; DAP-3; MRP-S29; bMRP-10; FLJ12817;
Gene ID : 7818
mRNA Refseq : NM_001199849
Protein Refseq : NP_001186778
MIM : 602074
UniProt ID : P51398

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