Recombinant Human DCLK1 Protein, GST-tagged

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Recombinant Human DCLK1 Protein, GST-tagged

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Cat.No. : DCLK1-2392H
Product Overview : Human DCLK1 (NP_004725.1, 1 a.a. - 729 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
Source : Insect Cells
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: GS peptide. ATP: 1000 uM
Molecular Mass : 108 kDa
Purity : 58 % by SDS-PAGE/CBB staining
Applications : Antibody Production
Functional Study
Compound Screening
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing: :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : DCLK1 doublecortin-like kinase 1 [ Homo sapiens ]
Official Symbol : DCLK1
Synonyms : DCLK1; doublecortin-like kinase 1; DCAMKL1, doublecortin and CaM kinase like 1; serine/threonine-protein kinase DCLK1; DCDC3A; DCLK; KIAA0369; doublecortin and CaM kinase-like 1; doublecortin-like and CAM kinase-like 1; doublecortin domain-containing protein 3A; CL1; CLICK1; DCAMKL1;
Gene ID : 9201
mRNA Refseq : NM_001195415
Protein Refseq : NP_001182344
MIM : 604742
UniProt ID : O15075

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