Recombinant Human DDR1 protein, His-tagged

• Cat.No.: DDR1-5688H
• Product Overview: Recombinant Human DDR1 aa. (His568~Phe829) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: His568~Phe829
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DDR1 discoidin domain receptor tyrosine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: DDR1
• Synonyms: DDR1; discoidin domain receptor tyrosine kinase 1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; epithelial discoidin domain-containing receptor 1; CD167 antigen-like family member A; PTK3A protein tyrosine kinase 3A; cell adhesion kinase; mammary carcinoma kinase 10; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; protein-tyrosine kinase RTK-6; tyrosine kinase DDR; tyrosine-protein kinase CAK
• Gene ID: 780
• mRNA Refseq: NM_001202521.1
• Protein Refseq: NP_001189450.1
• UniProt ID: Q08345