Recombinant Human DDX4 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human DDX4 Protein, Myc/DDK-tagged, C13 and N15-labeled

DDX4 Related Products

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Cat.No. : DDX4-2012H
Product Overview : DDX4 MS Standard C13 and N15-labeled recombinant protein (NP_001129506) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 79.3 kDa
AA Sequence : MGDEDWEAEI NPHMSSYVPI FEKDRYSGEN GDNFNRTPAS SSEMDDGPSR RDHFMKSGFA SGRNFGNRDA GECNKRDNTS TMGGFGVGKS FGNRGFSNSR FEDGDSSGFW RESSNDCEDN PTRNRGFSKR GGYRDGNNSE ASGPYRRGGR GSFRGCRGGF GLGSPNNDLD PDECMQRTGG LFGSRRPVLS GTGNGDTSQS RSGSGSERGG YKGLNEEVIT GSGKNSWKSE AEGGESSDTQ GPKVTYIPPP PPEDEDSIFA HYQTGINFDK YDTILVEVSG HDAPPAILTF EEANLCQTLN NNIAKAGYTK LTPVQKYSIP IILAGRDLMA CAQTGSGKTA AFLLPILAHM MHDGITASRF KELQEPECII VAPTRELVNQ IYLEARKFSF GTCVRAVVIY GGTQLGHSIR QIVQGCNILC ATPGRLMDII GKEKIGLKQI KYLVLDEADR MLDMGFGPEM KKLISCPGMP SKEQRQTLMF SATFPEEIQR LAAEFLKSNY LFVAVGQVGG ACRDVQQTVL QVGQFSKREK LVEILRNIGD ERTMVFVETK KKADFIATFL CQEKISTTSI HGDREQRERE QALGDFRFGK CPVLVATSVA ARGLDIENVQ HVINFDLPST IDEYVHRIGR TGRCGNTGRA ISFFDLESDN HLAQPLVKVL TDAQQDVPAW LEEIAFSTYI PGFSGSTRGN VFASVDTRKG KSTLNTAGFS SSQAPNPVDD ESWDTRTRPL EQKLISEEDL AANDILDYKD DDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : DDX4 DEAD-box helicase 4 [ Homo sapiens (human) ]
Official Symbol : DDX4
Synonyms : DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 4; probable ATP-dependent RNA helicase DDX4; VASA; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074;
Gene ID : 54514
mRNA Refseq : NM_001136034
Protein Refseq : NP_001129506
MIM : 605281
UniProt ID : Q9NQI0

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