Recombinant Human DEFA1B protein, His & GST-tagged

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Recombinant Human DEFA1B protein, His & GST-tagged

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Cat.No. : DEFA1B-1142H
Product Overview : Recombinant Human DEFA1B aa. (Glu20~Cys94 fused with N-terminal His & GST tag was produced in E. coli cells.
Description : Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene.
Source : E. coli
Species : Human
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : 42kDa as determined by SDS-PAGE reducing conditions.
Protein length : Glu20~Cys94
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >90%
Characteristic : The isoelectric point is 5.7.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : DEFA1B defensin alpha 1B [ Homo sapiens (human) ]
Official Symbol : DEFA1B
Synonyms : DEFA1B; defensin alpha 1B; HP1; HP-1; HNP-1; neutrophil defensin 1
Gene ID : 728358
mRNA Refseq : NM_001042500.1
Protein Refseq : NP_001035965.1
UniProt ID : P59665

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