Recombinant Human DENND1B Protein, GST-tagged

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Recombinant Human DENND1B Protein, GST-tagged

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Cat.No. : DENND1B-2533H
Product Overview : Human DENND1B full-length ORF ( NP_659414.1, 1 a.a. - 396 a.a.) recombinant protein with GST-tag at N-terminal.
Description : Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 71.4 kDa
AA Sequence : MAAAPREEKR WPQPVFSNPV VLWKFPEDFG DQEILQSVPK FCFPFDVERV SQNQVGQHFT FVLTDIESKQ RFGFCRLTSG GTICLCILSY LPWFEVYYKL LNTLADYLAK ELENDLNETL RSLYNHPVPK ANTPVNLSVH SYFIAPDVTG LPTIPESRNL TEYFVAVDVN NMLQLYASML HERRIVIISS KLSTLTACIH GSAALLYPMY WQHIYIPVLP PHLLDYCCAP MPYLIGIHSS LIERVKNKSL EDVVMLNVDT NTLESPFSDL NNLPSDVVSA LKNKLKKQST ATGDGVARAF LRAQAALFGY YRDALRYKPG EPITFCEESF VKHRSSVMKQ FLETAINLQL FKQFIDGRLA KLNAGRGFSD VFEEEITSGG FCGGKDKLQY DYPFSQ
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing: :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : DENND1B DENN/MADD domain containing 1B [ Homo sapiens ]
Official Symbol : DENND1B
Synonyms : DENND1B; DENN/MADD domain containing 1B; C1orf218, chromosome 1 open reading frame 218 , FAM31B, family with sequence similarity 31, member B; DENN domain-containing protein 1B; FLJ20054; MGC27044; connecdenn 2; family with sequence similarity 31, member B; FAM31B; C1ORF18; C1orf218; RP11-53I24.2; FLJ21179; FLJ33888; MGC103810; DKFZp547O0715;
Gene ID : 163486
mRNA Refseq : NM_001195215
Protein Refseq : NP_001182144
MIM : 613292
UniProt ID : Q6P3S1

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