Recombinant Human DENND1B Protein, GST-tagged

• Cat.No.: DENND1B-2533H
• Product Overview: Human DENND1B full-length ORF ( NP_659414.1, 1 a.a. - 396 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 71.4 kDa
• AA Sequence: MAAAPREEKRWPQPVFSNPVVLWKF PEDFGDQEILQSVPKFCFPFDVERV SQNQVGQHFTFVLTDIESKQRFGFC RLTSGGTICLCILSYLPWFEVYYKL LNTLADYLAKELENDLNETLRSLYN HPVPKANTPVNLSVHSYFIAPDVTG LPTIPESRNLTEYFVAVDVNNMLQL YASMLHERRIVIISSKLSTLTACIH GSAALLYPMYWQHIYIPVLPPHLLD YCCAPMPYLIGIHSSLIERVKNKSL EDVVMLNVDTNTLESPFSDLNNLPS DVVSALKNKLKKQSTATGDGVARAF LRAQAALFGYYRDALRYKPGEPITF CEESFVKHRSSVMKQFLETAINLQL FKQFIDGRLAKLNAGRGFSDVFEEE ITSGGFCGGKDKLQYDYPFSQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DENND1B DENN/MADD domain containing 1B [ Homo sapiens ]
• Official Symbol: DENND1B
• Synonyms: DENND1B; DENN/MADD domain containing 1B; C1orf218, chromosome 1 open reading frame 218 , FAM31B, family with sequence similarity 31, member B; DENN domain-containing protein 1B; FLJ20054; MGC27044; connecdenn 2; family with sequence similarity 31, member B; FAM31B; C1ORF18; C1orf218; RP11-53I24.2; FLJ21179; FLJ33888; MGC103810; DKFZp547O0715
• Gene ID: 163486
• mRNA Refseq: NM_001195215
• Protein Refseq: NP_001182144
• MIM: 613292
• UniProt ID: Q6P3S1