Recombinant Human DFFA Protein, GST-tagged

• Cat.No.: DFFA-2555H
• Product Overview: Human DFFA full-length ORF ( NP_004392.1, 1 a.a. - 331 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 62.9 kDa
• AA Sequence: MEVTGDAGVPESGEIRTLKPCLLRR NYSREQHGVAASCLEDLRSKACDIL AIDKSLTPVTLVLAEDGTIVDDDDY FLCLPSNTKFVALASNEKWAYNNSD GGTAWISQESFDVDETDSGAGLKWK NVARQLKEDLSSIILLSEEDLQMLV DAPCSDLAQELRQSCATVQRLQHTL QQVLDQREEVRQSKQLLQLYLQALE KEGSLLSKQEESKAAFGEEVDAVDT GISRETSSDVALASHILTALREKQA PELSLSSQDLELVTKEDPKALAVAL NWDIKKTETVQEACERELALRLQQT QSLHSLRSISASKASPPGDLQNPKR ARQDPT
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DFFA DNA fragmentation factor, 45kDa, alpha polypeptide [ Homo sapiens ]
• Official Symbol: DFFA
• Synonyms: DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, 45 kD, alpha polypeptide; DNA fragmentation factor subunit alpha; DFF 45; DFF1; DFF45; DNA fragmentation factor; 45 kD; alpha subunit; ICAD; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit; DFF-45
• Gene ID: 1676
• mRNA Refseq: NM_004401
• Protein Refseq: NP_004392
• MIM: 601882
• UniProt ID: O00273