Recombinant Human DFFB Protein, GST-tagged

• Cat.No.: DFFB-2557H
• Product Overview: Human DFFB full-length ORF ( NP_004393.1, 1 a.a. - 338 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 65.5 kDa
• AA Sequence: MLQKPKSVKLRALRSPRKFGVAGRS CQEVLRKGCLRFQLPERGSRLCLYE DGTELTEDYFPSVPDNAELVLLTLG QAWQGYVSDIRRFLSAFHEPQVGLI QAAQQLLCDEQAPQRQRLLADLLHN VSQNIAAETRAEDPPWFEGLESRFQ SKSGYLRYSCESRIRSYLREVSSYP STVGAEAQEEFLRVLGSMCQRLRSM QYNGSYFDRGAKGGSRLCTPEGWFS CQGPFDMDSCLSRHSINPYSNRESR ILFSTWNLDHIIEKKRTIIPTLVEA IKEQDGREVDWEYFYGLLFTSENLK LVHIVCHKKTTHKLNCDPSRIYKPQ TRLKRKQPVRKRQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DFFB DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) [ Homo sapiens ]
• Official Symbol: DFFB
• Synonyms: DFFB; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor subunit beta; CAD; CPAN; DFF 40; DFF40; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DFF2; DFF-40
• Gene ID: 1677
• mRNA Refseq: NM_004402
• Protein Refseq: NP_004393
• MIM: 601883
• UniProt ID: O76075