Recombinant Human DGAT1 protein, His-tagged

• Cat.No.: DGAT1-1652H
• Product Overview: Recombinant Human DGAT1 aa. (Ala180~Arg427) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala180~Arg427
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 10

Gene Information

• Gene Name: DGAT1 diacylglycerol O-acyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: DGAT1
• Synonyms: DGAT1; diacylglycerol O-acyltransferase 1; ARAT; DGAT; ARGP1; DIAR7; ACAT related gene product 1; acyl coenzyme A:cholesterol acyltransferase related gene 1; acyl-CoA retinol O-fatty-acyltransferase; acyl-CoA:diacylglycerol acyltransferase; diacylglycerol O-acyltransferase homolog; diglyceride acyltransferase
• Gene ID: 8694
• mRNA Refseq: NM_012079.5
• Protein Refseq: NP_036211.2
• UniProt ID: O75907