Recombinant Human DGKZ protein, His-tagged

• Cat.No.: DGKZ-3861H
• Product Overview: Recombinant Human DGKZ protein(297-601 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 297-601 aa
• AA Sequence: LLVFVNPKSGGNQGAKIIQSFLWYL NPRQVFDLSQGGPKEALEMYRKVHN LRILACGGDGTVGWILSTLDQLRLK PPPPVAILPLGTGNDLARTLNWGGG YTDEPVSKILSHVEEGNVVQLDRWD LHAEPNPEAGPEDRDEGATDRLPLD VFNNYFSLGFDAHVTLEFHESREAN PEKFNSRFRNKMFYAGTAFSDFLMG SSKDLAKHIRVVCDGMDLTPKIQDL KPKCVVFLNIPRYCAGTMPWGHPGE HHDFEPQRHDDGYLEVIGFTMTSLA ALQVGGHGERLTQCREVVLTTSKAI PVQVD
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: DGKZ diacylglycerol kinase, zeta [ Homo sapiens ]
• Official Symbol: DGKZ
• Synonyms: DGKZ; diacylglycerol kinase, zeta; diacylglycerol kinase, zeta 104kDa; diacylglycerol kinase zeta; DAGK5; DAGK6; DGK ZETA; hDGKzeta; DAG kinase zeta; diglyceride kinase zeta; DGK-ZETA
• Gene ID: 8525
• mRNA Refseq: NM_001105540
• Protein Refseq: NP_001099010
• MIM: 601441
• UniProt ID: Q13574