Recombinant Human DLL4 protein, His-Avi-tagged, Biotinylated

• Cat.No.: DLL4-3322H
• Product Overview: Biotinylated Recombinant Human DLL4 protein(Q9NR61)(Ser27-Pro524), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ser27-Pro524
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human DLL4, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Gene Name: DLL4 delta-like 4 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL4
• Synonyms: DLL4; delta-like 4 (Drosophila); delta like 4 homolog (Drosophila); delta-like protein 4; delta4; delta 4; delta ligand 4; notch ligand DLL4; delta-like 4 homolog; delta-like 4 protein; notch ligand delta-2; drosophila Delta homolog 4; hdelta2; MGC126344
• Gene ID: 54567
• mRNA Refseq: NM_019074
• Protein Refseq: NP_061947
• MIM: 605185
• UniProt ID: Q9NR61