Recombinant Human DMPK Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human DMPK Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : DMPK-5668H
Product Overview : DMPK MS Standard C13 and N15-labeled recombinant protein (NP_001075031) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-38 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 69.4 kDa
AA Sequence : MSAEVRLRRL QQLVLDPGFL GLEPLLDLLL GVHQELGASE LAQDKYVADF LQWAEPIVVR LKEVRLQRDD FEILKVIGRG AFSEVAVVKM KQTGQVYAMK IMNKWDMLKR GEVSCFREER DVLVNGDRRW ITQLHFAFQD ENYLYLVMEY YVGGDLLTLL SKFGERIPAE MARFYLAEIV MAIDSVHRLG YVHRDIKPDN ILLDRCGHIR LADFGSCLKL RADGTVRSLV AVGTPDYLSP EILQAVGGGP GTGSYGPECD WWALGVFAYE MFYGQTPFYA DSTAETYGKI VHYKEHLSLP LVDEGVPEEA RDFIQRLLCP PETRLGRGGA GDFRTHPFFF GLDWDGLRDS VPPFTPDFEG ATDTCNFDLV EDGLTAMETL SDIREGAPLG VHLPFVGYSY SCMALRDSEV PGPTPMELEA EQLLEPHVQA PSLEPSVSPQ DETAEVAVPA AVPAAEAEAE VTLRELQEAL EEEVLTRQSL SREMEAIRTD NQNFASQLRE AEARNRDLEA HVRQLQERME LLQAEGATAV TGVPSPRATD PPSHMAPRPW LWASARWWGQ APCTAATCCS LPGSLGLAYR RRFPCSCSPL FCLVPPPWAA LGWWPTPANS PQSGAAQEPP ALPEPTRTRP LEQKLISEED LAANDILDYK DDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : DMPK dystrophia myotonica-protein kinase [ Homo sapiens (human) ]
Official Symbol : DMPK
Synonyms : DMPK; dystrophia myotonica-protein kinase; myotonin-protein kinase; DM; DM protein kinase; DM1; DM1PK; DMK; dystrophia myotonica 1; MDPK; MT PK; myotonic dystrophy associated protein kinase; myotonin protein kinase A; thymopoietin homolog; DM-kinase; DM1 protein kinase; myotonic dystrophy protein kinase; MT-PK;
Gene ID : 1760
mRNA Refseq : NM_001081562
Protein Refseq : NP_001075031
MIM : 605377
UniProt ID : Q09013

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