Recombinant Human DNA-damage-inducible Transcript 3

• Cat.No.: DDIT3-130H
• Product Overview: Recombinant Human DNA-damage-inducible transcript 3 protein expressed inE.coliencodes full length amino acids and has a molecular mass of 52 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. GADD153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and merabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites.
• Formulation: Recombinant Human GADD153 protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application: •ELISA •Inhibition Assays •Western Blotting.
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 52 kDa.
• Storage & Stability: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.
• Full Length: Full L.
• Publications:
  Is there a common upstream link for autophagic and apoptotic cell death in human high-grade gliomas? (2011)

Gene Information

• Gene Name: DDIT3 DNA-damage-inducible transcript 3 [ Homo sapiens ]
• Synonyms: DDIT3; DNA-damage-inducible transcript 3; CHOP; CEBPZ; CHOP10; GADD153; MGC4154; C/EBP zeta; DDIT-3; Growth arrest and DNA-damage-inducible protein GADD153; C/EBP-homologous protein; C/EBP homologous protein; CCAAT/enhancer-binding protein homologous protein; growth arrest- and DNA damage-inducible
• Gene ID: 1649
• mRNA Refseq: NM_004083
• Protein Refseq: NP_004074
• MIM: 126337
• UniProt ID: P35638
• Chromosome Location: 12q13.1-q13.2
• Pathway: MAPK signaling pathway; Diabetes pathways
• Function: protein dimerization activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity

Citation

Is there a common upstream link for autophagic and apoptotic cell death in human high-grade gliomas?

Journal: Neuro-Oncology    PubMed ID: 21727211    Data: 2011/7/1

Authors: Luni Emdad, Zulekha A. Qadeer, Isabelle M. Germano

Article Snippet:8 , 9 Cisplatin (CDDP) (Enzo Life Sciences) was used at a concentration of 5 μg/mL in all experiments.8 , 9 Cisplatin (CDDP) (Enzo Life Sciences) was used at a concentration of 5 μg/mL in all experiments.. 34 Recombinant human DNA damage–inducible transcript 3/GADD153 (Creative BioMart) was used at a concentration of 1 μg/mL.. CXB (Toronto Research Chemicals) was used at concentrations of 10 and 20 μM.CXB (Toronto Research Chemicals) was used at concentrations of 10 and 20 μM.

Cell viability, apoptosis, and autophagy rate in human HGG cells after GADD153 complementary DNA overexpression. Bar graphs showing a significant decrease in cell viability after overexpression of GADD153 complementary DNA in U87 (A) and T98G (E) HGG cells. A significant increase in apoptotic (B and F) and autophagic (C and G) rate was also seen. *P < .05, Student's t-test compared with vector. Western blotting showing increased expression of CHOP/GADD153, apoptosis (cleaved caspase-3), and autophagy (LC3II) markers in U87 (D) and T98G (H) cells after transient transfection of CHOP/GADD153.