Recombinant Human DNA-Damage-Inducible Transcript 3, His-tagged

• Cat.No.: DDIT3-4948H
• Product Overview: Recombinant GADD153 fused with His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: GADD153, also known as DNA-damage-inducible transcript 3 (DDIT3), is a basic domain-leucine zipper(bZIP) transcription factor of C/EBP family. This protein has been shown to be up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. GADD153 protein may play a role in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease.
• Concentration: 1 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 50mM NaCl and 10% glycerol.
• Purity: > 90 by SDS - PAGE
• Sequence: 1-169 amino acids: MGSSHHHHHH SSGLVPRGSH MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA
• Molecular Mass: 21.3 kDa (189aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: DDIT3 DNA-damage-inducible transcript 3 [ Homo sapiens ]
• Official Symbol: DDIT3
• Synonyms: DDIT3; DNA-damage-inducible transcript 3; CHOP; CEBPZ; CHOP10; GADD153; MGC4154; C/EBP zeta; DDIT-3; Growth arrest and DNA-damage-inducible protein GADD153; C/EBP-homologous protein; C/EBP homologous protein; CCAAT/enhancer-binding protein homologous protein; growth arrest- and DNA damage-inducible
• Gene ID: 1649
• mRNA Refseq: NM_004083
• Protein Refseq: NP_004074
• MIM: 126337
• UniProt ID: P35638
• Chromosome Location: 12q13.1-q13.2
• Pathway: MAPK signaling pathway; Diabetes pathways
• Function: protein dimerization activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity