Recombinant Human DNAAF1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DNAAF1-855H
• Product Overview: LRRC50 MS Standard C13 and N15-labeled recombinant protein (NP_848547) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 80 kDa
• AA Sequence: MHPEPSEPATGGAAELDCAQEPGVE ESAGDHGSAGRGGCKEEINDPKEIC VGSSDTSYHSQQKQSGDNGSGGHFA HPREDREDRGPRMTKSSLQKLCKQH KLYITPALNDTLYLHFKGFDRIENL EEYTGLRCLWLQSNGIQKIENLEAQ TELRCLFLQMNLLRKIENLEPLQKL DALNLSNNYIKTIENLSCLPVLNTL QMAHNHLETVEDIQHLQECLRLCVL DLSHNKLSDPEILSILESMPDLRVL NLMGNPVIRQIPNYRRTVTVRLKHL TYLDDRPVFPKDRACAEAWARGGYA AEKEERQQWESRERKKITDSIEALA MIKQRAEERKRQRESQERGEMTSSD DGENVPASAEGKEEPPGDRETRQKM ELFVKESFEAKDELCPERPSGEEPP VEAKREDGGPEPEGTLPAETLLLSS PVEVKGEDGDGEPEGTLPAEAPPPP PPVEVKGEDGDQEPEGTLPAETLLL SPPVKVKGEDGDREPEGTLPAEAPP PLPLGAAREEPTPQAVATEGVFVTE LDGTRTEDLETIRLETKETCCIDDL PDLEDDDETGKSLEDQNMCFPKIEV ISSLSDDSDPELDYTSLPVLENLPT DTLSNIFAVSKDTSKAARVPFTDIF KKEAKRDSEIRKQDTKSPRPLIQEL SDEDPSGQPLMPPTCQRDAAPLTST GDRDSDFLAASSPVPTESAATPPET CVGVAQPSQALPTWDLTAFPAPKAS TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DNAAF1 dynein axonemal assembly factor 1 [ Homo sapiens (human) ]
• Official Symbol: DNAAF1
• Synonyms: DNAAF1; dynein, axonemal, assembly factor 1; leucine rich repeat containing 50, LRRC50; dynein assembly factor 1, axonemal; CILD13; FLJ25330; ODA7; outer row dynein assembly 7 homolog (Chlamydomonas); leucine rich repeat containing 50; outer row dynein assembly 7 homolog; leucine-rich repeat-containing protein 50; LRRC50; DKFZp434A119
• Gene ID: 123872
• mRNA Refseq: NM_178452
• Protein Refseq: NP_848547
• MIM: 613190
• UniProt ID: Q8NEP3