Recombinant Human DNASE1 protein

• Cat.No.: DNASE1-10H
• Product Overview: Recombinant human deoxyribonuclease I (a glycosylated, polypeptide chain containing 260 amino acids) without tag was produced in CHO. DNase is purified by proprietary chromatographic techniques. Specific activity is 1000IU/1mg.

Specification

• Description: This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
• Source: CHO Cell
• Species: Human
• Form: Sterile liquid colorless solution
• Molecular Mass: 37 kDa
• Unit Definition: Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260 nm of 0.001/minute at 25 centigrade when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1µg of lambda phage DNA in 10 minutes at 37 centigrade in 50mM Tris, 1mMMg++, pH 7.8 in a 50 μL reaction.
• Notes: This product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Stability: 2 years when stored at 4 centigrade, three weeks at 15 centigrade, pH-6.3.
• Concentration: 1 mg/mL
• Storage Buffer: Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease 1 [ Homo sapiens (human) ]
• Official Symbol: DNASE1
• Synonyms: EC 3.1.21.1; Deoxyribonuclease I; DNase I; DNL1; DRNI; FLJ38093; DNASE1; deoxyribonuclease-1; DNase I, lysosomal; Dornase alfa; deoxyribonuclease I; human urine deoxyribonuclease I; EC 3.1.21.1
• Gene ID: 1773
• mRNA Refseq: NM_005223
• Protein Refseq: NP_005214
• MIM: 125505
• UniProt ID: P24855