Recombinant Human DNMT1 Protein, His-Flag-tagged

• Cat.No.: DNMT1-330H
• Product Overview: Full-length Recombinant Human DNMT1 Protein (residues 2-1616 (C-terminus); Genbank Accession # NM_001379) expressed in SF9 Insect Cells with an N-terminal His-tag and a C-terminal Flag-Tag.

Specification

• Description: DNA methylation is recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. DNA (cytosine-5)-methyltransferase1 (DNMT1) preferentially methylates hemi-methylated DNA, in a CpG context, to maintain the methylation pattern in the new strand of replicated DNA. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.
• Source: Insect Cells
• Species: Human
• Tag: His-Flag
• Form: 25 μg recombinant DNMT1 protein supplied at a concentration of 0.5 μg/μl in 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 100mM EDTA pH 8, 0.01% NP-40, and 10% glycerol.
• Molecular Mass: 182 kDa
• AA Sequence: MHHHHHHLEPARTAPARVPTLAVPA ISLPDDVRRRLKDLERDSLTEKECV KEKLNLLHEFLQTEIKNQLCDLETK LRKEELSEEGYLAKVKSLLNKDLSL ENGAHAYNREVNGRLENGNQARSEA RRVGMADANSPPKPLSKPRTPRRSK SDGEAKPEPSPSPRITRKSTRQTTI TSHFAKGPAKRKPQEESERAKSDES IKEEDKDQDEKRRRVTSRERVARPL PAEEPERAKSGTRTEKEEERDEKEE KRLRSQTKEPTPKQKLKEEPDREAR AGVQADEDEDGDEKDEKKHRSQPKD LAAKRRPEEKEPEKVNPQISDEKDE DEKEEKRRKTTPKEPTEKKMARAKT VMNSKTHPPKCIQCGQYLDDPDLKY GQHPPDAVDEPQMLTNEKLSIFDAN ESGFESYEALPQHKLTCFSVYCKHG HLCPIDTGLIEKNIELFFSGSAKPI YDDDPSLEGGVNGKNLGPINEWWIT GFDGGEKALIGFSTSFAEYILMDPS PEYAPIFGLMQEKIYISKIVVEFLQ SNSDSTYEDLINKIETTVPPSGLNL NRFTEDSLLRHAQFVVEQVESYDEA GDSDEQPIFLTPCMRDLIKLAGVTL GQRRAQARRQTIRHSTREKDRGPTK ATTTKLVYQIFDTFFAEQIEKDDRE DKENAFKRRRCGVCEVCQQPECGKC KACKDMVKFGGSGRSKQACQERRCP NMAMKEADDDEEVDDNIPEMPSPKK MHQGKKKKQNKNRISWVGEAVKTDG KKSYYKKVCIDAETLEVGDCVSVIP DDSSKPLYLARVTALWEDSSNGQMF HAHWFCAGTDTVLGATSDPLELFLV DECEDMQLSYIHSKVKVIYKAPSEN WAMEGGMDPESLLEGDDGKTYFYQL WYDQDYARFESPPKTQPTEDNKFKF CVSCARLAEMRQKEIPRVLEQLEDL DSRVLYYSATKNGILYRVGDGVYLP PEAFTFNIKLSSPVKRPRKEPVDED LYPEHYRKYSDYIKGSNLDAPEPYR IGRIKEIFCPKKSNGRPNETDIKIR VNKFYRPENTHKSTPASYHADINLL YWSDEEAVVDFKAVQGRCTVEYGED LPECVQVYSMGGPNRFYFLEAYNAK SKSFEDPPNHARSPGNKGKGKGKGK GKPKSQACEPSEPEIEIKLPKLRTL DVFSGCGGLSEGFHQAGISDTLWAI EMWDPAAQAFRLNNPGSTVFTEDCN ILLKLVMAGETTNSRGQRLPQKGDV EMLCGGPPCQGFSGMNRFNSRTYSK FKNSLVVSFLSYCDYYRPRFFLLEN VRNFVSFKRSMVLKLTLRCLVRMGY QCTFGVLQAGQYGVAQTRRRAIILA AAPGEKLPLFPEPLHVFAPRACQLS VVVDDKKFVSNITRLSSGPFRTITV RDTMSDLPEVRNGASALEISYNGEP QSWFQRQLRGAQYQPILRDHICKDM SALVAARMRHIPLAPGSDWRDLPNI EVRLSDGTMARKLRYTHHDRKNGRS SSGALRGVCSCVEAGKACDPAARQF NTLIPWCLPHTGNRHNHWAGLYGRL EWDGFFSTTVTNPEPMGKQGRVLHP EQHRVVSVRECARSQGFPDTYRLFG NILDKHRQVGNAVPPPLAKAIGLEI KLCMLAKARESASAKIKEEEAAKDA AADYKDDDDK*
• Purity: Purified using FPLC, >98% purity as determined by SDS-PAGE.
• Applications: Recombinant DNMT1 is suitable for protein-protein interaction studies, DNA methylation assays, drug screening, structural studies, etc.
• Notes: This product is for research use only. Not for use in diagnostic or therapeutic procedures.
• Stability: Guaranteed stable for 6 months from date of receipt when stored properly.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.5 µg/µl

Gene Information

• Gene Name: DNMT1 DNA (cytosine-5-)-methyltransferase 1 [ Homo sapiens ]
• Official Symbol: DNMT1
• Synonyms: DNMT1; DNA (cytosine-5-)-methyltransferase 1; DNMT; CXXC9; MCMT; m.HsaI; DNA MTase HsaI; AIM; HSN1E; FLJ16293; MGC104992
• Gene ID: 1786
• mRNA Refseq: NM_001130823
• Protein Refseq: NP_001124295
• MIM: 126375
• UniProt ID: P26358