Recombinant Human DOHH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human DOHH Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : DOHH-6406H
Product Overview : DOHH MS Standard C13 and N15-labeled recombinant protein (NP_112594) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 32.7 kDa
AA Sequence : MVTEQEVDAI GQTLVDPKQP LQARFRALFT LRGLGGPGAI AWISQAFDDD SALLKHELAY CLGQMQDARA IPMLVDVLQD TRQEPMVRHE AGEALGAIGD PEVLEILKQY SSDPVIEVAE TCQLAVRRLE WLQQHGGEPA AGPYLSVDPA PPAEERDVGR LREALLDESR PLFERYRAMF ALRNAGGEEA ALALAEGLHC GSALFRHEVG YVLGQLQHEA AVPQLAAALA RCTENPMVRH ECAEALGAIA RPACLAALQA HADDPERVVR ESCEVALDMY EHETGRAFQY ADGLEQLRGA PSTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : DOHH deoxyhypusine hydroxylase/monooxygenase [ Homo sapiens (human) ]
Official Symbol : DOHH
Synonyms : DOHH; deoxyhypusine hydroxylase/monooxygenase; HEAT like (PBS lyase) repeat containing 1, HLRC1; deoxyhypusine hydroxylase; MGC4293; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1; HEAT-like (PBS lyase) repeat containing 1; HLRC1; hDOHH;
Gene ID : 83475
mRNA Refseq : NM_031304
Protein Refseq : NP_112594
MIM : 611262
UniProt ID : Q9BU89

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