Recombinant Human DPEP2, His tagged

• Cat.No.: DPEP2-8611H
• Product Overview: Recombinant Human DPEP2 (AAH24021.1) (Met1-Ser376), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: Human Cells
• Tag: Non
• Protein Length: 1-376 a.a.
• Predicted N Terminal: Ala 33
• Form: Lyophilized from sterile PBS, pH 7.4Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human DPEP2 consists of 355 amino acids and predicts a molecular mass of 39.4 KDa. It migrates as an approximately 47 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: DPEP2 dipeptidase 2 [ Homo sapiens ]
• Official Symbol: DPEP2
• Gene ID: 64174
• mRNA Refseq: NM_022355
• Protein Refseq: NP_071750
• MIM: 609925
• UniProt ID: Q9H4A9
• Chromosome Location: 16q22.1

Citation

Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.

Journal: Cell    PubMed ID: 31442408    Data: 2020/5/11

Authors: Saurav Roy Choudhury, Liane Babes, Donna L Senger

Article Snippet:REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-b-actin (Clone C4) Millipore Cat# MAB1501; RRID: AB_2223041 Rat anti-CD31 (Clone 390) eBioscience Cat# 14-0311-81; RRID: AB_467200 AF647 Rat anti-mouse CD31 (Clone 390) eBioscience Cat# 14-0311-82; RRID: AB_467201 AF647 Rat anti-mouse CD31 (Clone 390) BioLegend Cat# 102515; RRID: AB_2161030 AF488 Rat anti-mouse CD31 (Clone 390) BioLegend Cat# 102513; RRID: AB_493413 Rabbit anti-human/mouse DPEP1 Proteintech Cat# 12222-1-AP Rabbit anti-human DPEP1 Sigma Cat# HPA012783; RRID: AB_1847843 Rabbit anti-human DPEP1 Abcam Cat# ab121308; RRID: AB_11133237 Rabbit anti-human DPEP2 Abcam Cat# ab125516; RRID: AB_11129101 Goat anti-human DPEP3 (Clone N-16) Santa Cruz Biotechnology Cat# sc-164219; RRID: AB_10846044 Rat anti-mouse Gr-1 (Clone RB6-8C5) BioXcell Cat# BE0075; RRID: AB_10312146 Mouse anti-6X His tag (Clone HIS.H8) Thermo Scientific Cat# MA1-21315; RRID: AB_557403 Rat anti-mouse LY6G (Clone 1A8) Biolegend Cat# 127601/127602; RRID: AB_1089179; RRID: AB_1089180 AF 594 Rat anti-mouse LY6G (Clone 1A8) Biolegend Cat# 127636; RRID: AB_2563207 Rat anti-mouse F4/80 (Clone BM8) eBioscience Cat# 14-4801-82; RRID: AB_467558 Alexa Fluor 488–conjugated goat anti-mouse lgG Invitrogen Cat# R37120; RRID: AB_2556548 Goat-anti mouse secondary antibody, HRP (For Western Blot analysis) Santa Cruz Biotechnology Cat# sc-2055; RRID: AB_631738 Goat-anti-rabbit secondary antibody, HRP (For Western Blot analysis) Santa Cruz Biotechnology Cat# sc-2054; RRID: AB_631748 Goat anti-mouse secondary antibody HRP (For IHC) Dako Cat# K4007 Goat anti-rabbit secondary antibody HRP (For IHC) Dako Cat# K4011 Goat anti-rat secondary antibody, Biotinylated (For IHC) Vector Laboratories Cat# BA-9401; RRID: AB_2336208 Bacterial and Virus Strains T7Select 10-3b Phage Display Cloning Kit Novagen Cat# 70550-3 Biological Samples Kidney tissues were obtained from non-diseased margins of human nephrectomies Alberta Health Services NA Human lung explants International Institute for Advancement of Medicine NA Chemicals, Peptides, and Recombinant Proteins LSALT peptide, C-term amidated (H-LSALTPSPSW LKYKAL-NH2) This paper (CanPeptide) NA Scrambled LSALT peptide, C-term amidated (H-LWLPLKSATPSYALSK-NH2) This paper (CanPeptide) NA KGAL peptide, C-term amidated (H-KKGALWGRQK QHQGCLGEDWWPWC-NH2) This paper (CanPeptide) NA GFE1 peptide, C-term amidated (H-CGFECVRQCPE RC-NH2) Rajotte and Ruoslahti, 1999 NA GFE2 peptide, C-term amidated (H-CGFELETC-NH2) Rajotte and Ruoslahti, 1999 NA Recombinant Human DPEP1, His tagged Creative BioMart DPEP1-77H Recombinant Human DPEP2, His tagged Creative BioMart DPEP2-8611H (Continued on next page) e1 Cell 178, 1205–1221.e1–e10, August 22, 2019