Recombinant Human DPEP2 protein(Met1-Ser376), hFc-tagged

• Cat.No.: DPEP2-8610H
• Product Overview: Recombinant Human DPEP2 (AAH24021.1) (Met1-Ser376) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ser376
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human DPEP2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 585 amino acids and has a predicted molecular mass of 65 kDa. The apparent molecular mass of the protein is approximately 71.9 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DPEP2 dipeptidase 2 [ Homo sapiens ]
• Official Symbol: DPEP2
• Synonyms: DPEP2; dipeptidase 2; MBD2
• Gene ID: 64174
• mRNA Refseq: NM_022355
• Protein Refseq: NP_071750
• MIM: 609925
• UniProt ID: Q9H4A9

Citation

Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.

Journal: Cell    PubMed ID: 31442408    Data: 2020/5/11

Authors: Saurav Roy Choudhury, Liane Babes, Donna L Senger

Article Snippet:Proteins bound to each peptide were resolved by SDS-PAGE and bound proteins were detected by western blot analysis using anti-His (Thermo Scientific).Proteins bound to each peptide were resolved by SDS-PAGE and bound proteins were detected by western blot analysis using anti-His (Thermo Scientific).. Adhesion of Human Neutrophils to recombinant DPEP1 in vitro Recombinant human DPEP1 or DPEP2 with 6x His tag at the C-terminal ends were purchased from Creative BioMart and reconstituted in ddH2O (1 mg/ml in PBS; salts were included in lyophilized buffer).. The DPEP1 stock solution was diluted to 0.45 pmol/ml (mM) in DPBS, and 40 ml (18 pmol) of this solution (or PBS or DPEP2 controls) was added to each well of a 96-well format nickel coated plate, and incubated at 4 C overnight.The DPEP1 stock solution was diluted to 0.45 pmol/ml (mM) in DPBS, and 40 ml (18 pmol) of this solution (or PBS or DPEP2 controls) was added to each well of a 96-well format nickel coated plate, and incubated at 4 C overnight.