Recombinant Human DPP4 Protein, His-tagged

• Cat.No.: DPP4-116H
• Product Overview: Recombinant human DPP4 protein(39-766aa) with His tag was expressed in insect cell.

Specification

• Description: The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 85.4kDa
• AA Sequence: ADPSRKTYTLTDYLKNTYRLKLYSL RWISDHEYLYKQENNILVFNAEYGN SSVFLENSTFDEFGHSINDYSISPD GQFILLEYNYVKQWRHSYTASYDIY DLNKRQLITEERIPNNTQWVTWSPV GHKLAYVWNNDIYVKIEPNLPSYRI TWTGKEDIIYNGITDWVYEEEVFSA YSALWWSPNGTFLAYAQFNDTEVPL IEYSFYSDESLQYPKTVRVPYPKAG AVNPTVKFFVVNTDSLSSVTNATSI QITAPASMLIGDHYLCDVTWATQER ISLQWLRRIQNYSVMDICDYDESSG RWNCLVARQHIEMSTTGWVGRFRPS EPHFTLDGNSFYKIISNEEGYRHIC YFQIDKKDCTFITKGTWEVIGIEAL TSDYLYYISNEYKGMPGGRNLYKIQ LSDYTKVTCLSCELNPERCQYYSVS FSKEAKYYQLRCSGPGLPLYTLHSS VNDKGLRVLEDNSALDKMLQNVQMP SKKLDFIILNETKFWYQMILPPHFD KSKKYPLLLDVYAGPCSQKADTVFR LNWATYLASTENIIVASFDGRGSGY QGDKIMHAINRRLGTFEVEDQIEAA RQFSKMGFVDNKRIAIWGWSYGGYV TSMVLGSGSGVFKCGIAVAPVSRWE YYDSVYTERYMGLPTPEDNLDHYRN STVMSRAENFKQVEYLLIHGTADDN VHFQQSAQISKALVDVGVDFQAMWY TDEDHGIASSTAHQHIYTHMSHFIK QCFSLPHHHHHH
• Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280 nm)
• Storage Buffer: Liquid in 20mM Tris-HCl pH 8.0, 100mM NaCl, 1mM EDTA, 10% glycerol
• Protein length: 39-766

Gene Information

• Gene Name: DPP4 dipeptidyl peptidase 4 [ Homo sapiens (human) ]
• Official Symbol: DPP4
• Synonyms: CD26; ADABP; ADCP2; DPPIV; TP103; dipeptidyl peptidase 4; ADCP-2; DPP IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); EC 3.4.14.5
• Gene ID: 1803
• mRNA Refseq: NM_001935
• Protein Refseq: NP_001926
• MIM: 102720
• UniProt ID: P27487

Citation

Peptide Array on Cellulose Support—A Screening Tool to Identify Peptides with Dipeptidyl-Peptidase IV Inhibitory Activity within the Sequence of α-Lactalbumin

Journal: International Journal of Molecular Sciences    PubMed ID: 25402645    Data: 2014/11/13

Authors: Isabelle M. E. Lacroix, Eunice C. Y. Li-Chan, Brijesh Tiwari

Article Snippet:Recombinant human dipeptidyl-peptidase IV (DPP-IV, EC 3.4.14.5, expressed in HEK293 human cells, >95% purity, activity >5500 pmoles Gly–Pro–7-amido-4-methylcoumarin/min/μg) was purchased from Creative BioMart (Shirley, NY, USA) while polyclonal goat DPP-IV antibody and polyclonal donkey anti-goat secondary antibody conjugated to horseradish peroxidase were obtained from R&D Systems (Minneapolis, MN, USA).. Pierce? ECL western blotting substrate and protein-free (TBS) blocking buffer were from Thermo Scientific (Rockford, IL, USA).Pierce? ECL western blotting substrate and protein-free (TBS) blocking buffer were from Thermo Scientific (Rockford, IL, USA).

Schematic representation of peptide array synthesis, binding and inhibition experiments. Binding of α-lactalbumin-derived decamers to the dipeptidyl-peptidase IV (DPP-IV) enzyme was investigated directly on the cellulose membrane, whereas the inhibition of DPP-IV was measured using a colorimetric assay with the cleaved peptides.

Binding of DPP-IV to deca-peptides derived from α-lactalbumin (A1–K4, ) and from other proteins (K5–K11, and ). The binding is inversely correlated to the light signal, the darkest spots representing peptides on which more enzyme was bound.

Correlation between binding of DPP-IV to deca-peptides on the array (indicated by luminescence intensity) and inhibition of the enzyme activity by the peptides.

Reviews

09/13/2019

Its purity and efficacy make it an ideal choice for precise and accurate analysis, allowing me to generate reliable results.

01/27/2019

By incorporating the DPP4 Protein into their studies, scientists can confidently conduct their research, ushering in groundbreaking discoveries and novel insights within their respective domains.

12/14/2016

Its exceptional purity and functionality make it an ideal choice for accurate and precise analysis, ensuring reliable and reproducible results.

Q&As

How does DPP4 relate to diabetes?

DPP4 plays a crucial role in glucose homeostasis by cleaving incretin hormones. In diabetes, inhibiting DPP4 can enhance the action of these hormones, promoting insulin release and lowering blood sugar levels.

Are there any side effects associated with DPP4 inhibitors?

Common side effects of DPP4 inhibitors include respiratory and urinary tract infections, headache, and nasopharyngitis. However, they are generally well-tolerated.

What clinical applications does DPP4 have in managing type 2 diabetes?

Inhibitors of DPP4 are used in the treatment of type 2 diabetes to improve glycemic control. They work by prolonging the activity of incretin hormones, such as GLP-1, which stimulate insulin secretion.

Can DPP4 inhibitors be used as a monotherapy for diabetes?

DPP4 inhibitors are often used as monotherapy or in combination with other antidiabetic medications to achieve optimal glycemic control.

How do DPP4 inhibitors differ from other diabetes medications?

DPP4 inhibitors work by enhancing the effects of incretin hormones, while other diabetes medications may act on insulin sensitivity, insulin release, or glucose absorption.