Recombinant Human E2F2 protein, His & GST-tagged

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Recombinant Human E2F2 protein, His & GST-tagged

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Cat.No. : E2F2-1635H
Product Overview : Recombinant Human E2F2 aa. (Arg129~Tyr429 (Accession # Q14209)) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.
Source : E. coli
Species : Human
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : 68kDa as determined by SDS-PAGE reducing conditions.
Protein length : Arg129~Tyr429 (Accession # Q14209)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >90%
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Isoelectric Point : 4.4
SDS-PAGE :

Gene Name : E2F2 E2F transcription factor 2 [ Homo sapiens (human) ]
Official Symbol : E2F2
Synonyms : E2F2; E2F transcription factor 2; E2F-2; transcription factor E2F2
Gene ID : 1870
mRNA Refseq : NM_004091.3
Protein Refseq : NP_004082.1
UniProt ID : Q14209
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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