Recombinant Human Ebi3&Il27 protein, His-tagged

• Cat.No.: Ebi3&Il27-1435H
• Product Overview: Recombinant Human Ebi3&Il27 protein(Q14213(Ebi3)&Q8NEV9(Il27))(Arg21-Lys229(Ebi3) & Phe29-Pro243(Il27)), fused with C-terminal His tag, was expressed in CHO.

Specification

• Species: Human
• Source: C-His
• Protein Length: Arg21-Lys229(Ebi3) & Phe29-Pro243(Il27)
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS, 200mM L-Arginine (pH 7.4).
• Bio-activity: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EBI3 Epstein-Barr virus induced 3 [ Homo sapiens ]
• Official Symbol: Ebi3
• Synonyms: EBI3; Epstein-Barr virus induced 3; interleukin-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; IL-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein; IL27B; IL-27B
• Gene ID: 10148
• mRNA Refseq: NM_005755
• Protein Refseq: NP_005746
• MIM: 605816
• UniProt ID: Q14213
• Gene Name 2: IL27 interleukin 27 [ Homo sapiens ]
• Official Symbol 2: Il27
• Synonyms 2: IL27; interleukin 27
• Gene ID 2: 144841