Recombinant Human EBI3/p28 protein, His-tagged EBI3-256H

Recombinant Human EBI3/p28 protein, His-tagged

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Recombinant Human EBI3/p28 protein, His-tagged

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Cat.No. : EBI3-256H
Product Overview : Recombinant Human EBI3(Arg21-Lys229) and p28(Phe29-Pro243) fused with His tag at C-terminal was expressed in NS0.
Description : Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSxWS-like motif that places the IL-27 molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes.Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four α-helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and pro-inflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development).
Source : NS0
Species : Human
Tag : His
Predicted N Terminal : Arg21
Form : Supplied as a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS.
Bio-activity : Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is typically 1-8 ng/mL.
Molecular Mass : Predicted Molecular Mass: 50 kDa;SDS-PAGE: 60 kDa, reducing conditions
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Stability : Avoid repeated freeze­thaw cycles.12 months from date of receipt, - ­70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after opening.3 months, - ­20 to ­- 70 centigrade under sterile conditions after opening.
Gene Name : EBI3 Epstein-Barr virus induced 3 [ Homo sapiens ]
Official Symbol : EBI3
Synonyms : EBI3; Epstein-Barr virus induced 3; interleukin-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; IL-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein; IL27B; IL-27B;
Gene ID : 10148
mRNA Refseq : NM_005755
Protein Refseq : NP_005746
MIM : 605816
UniProt ID : Q14213
Chromosome Location : 19p13
Pathway : IL27-mediated signaling events, organism-specific biosystem;
Function : cytokine activity; cytokine receptor activity; interleukin-27 receptor binding;

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