Recombinant Human ECD protein(Met1-Thr621), His&GST-tagged

• Cat.No.: ECD-1317H
• Product Overview: Recombinant Human ECD (O95905-1) (Met1-Thr621) was expressed in Insect Cells with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Met1-Thr621
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Human ECD/GST chimera consists of 881 amino acids and has a calculated molecular mass of 100.6 kDa. The recombinant protein migRates as an approximately 88-108 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ECD ecdysoneless homolog (Drosophila) [ Homo sapiens ]
• Official Symbol: ECD
• Synonyms: ECD; ecdysoneless homolog (Drosophila); protein SGT1; GCR2; hSGT1; suppressor of GCR2; protein ecdysoneless homolog; suppressor of S. cerevisiae gcr2; HSGT1
• Gene ID: 11319
• mRNA Refseq: NM_001135752
• Protein Refseq: NP_001129224
• UniProt ID: O95905