Recombinant Human EFNA5 protein, His & T7-tagged

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Recombinant Human EFNA5 protein, His & T7-tagged

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Cat.No. : EFNA5-1133H
Product Overview : Recombinant Human EFNA5 aa. (Gln21~Asn203 (Accession # P52803) fused with N-terminal His & T7 tag was produced in E. coli cells.
Description : Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 24.7kDa.
Protein length : Gln21~Asn203 (Accession # P52803)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 6.5.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE :

Gene Name : EFNA5 ephrin A5 [ Homo sapiens (human) ]
Official Symbol : EFNA5
Synonyms : EFNA5; ephrin A5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7; ephrin-A5; AL-1; LERK-7; eph-related receptor tyrosine kinase ligand 7
Gene ID : 1946
mRNA Refseq : NM_001962.2
Protein Refseq : NP_001953.1
UniProt ID : P52803
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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