Recombinant Human EGFP protein

• Cat.No.: EGFP-411H
• Product Overview: Recombinant Human EGFP protein( P42212) was expressed in E. coli.

Specification

• Species: Human
• Source: E. coli
• Tag: Non
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
• Molecular Mass: Approximately 26.9 kDa, a single non-glycosylated polypeptide chain containing 239 amino acids.
• AASequence: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK
• Endotoxin: Less than 1 EU/µg of reGFP as determined by LAL method.
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.