Active Recombinant Human EGFRvIII Protein, His & Avi-tagged, Biotinylated
Cat.No. : | EGFR-341H |
Product Overview : | Recombinant Human EGFRvIII Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly) (Accession # NP_005219.2). Predicted N-terminus: Leu 25. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing (R) condition, and 60-70 kDa under non-reducing (NR) condition (SDS-PAGE). The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized CetuxiMabat 2 μg/mL (100 μL/well) can bind Recombinant Human EGFRvIII Protein, His & Avi-tagged, Biotinylated with a linear range of 0.4-25 ng/mL (QC tested). |
Molecular Mass : | 41.3 kDa |
Protein length : | Leu 25 - Ser 645 |
AA Sequence : | LEEKKGNYVVTDHGSCVRACGADSY EMEEDGVRKCKKCEGPCRKVCNGIG IGEFKDSLSINATNIKHFKNCTSIS GDLHILPVAFRGDSFTHTPPLDPQE LDILKTVKEITGFLLIQAWPENRTD LHAFENLEIIRGRTKQHGQFSLAVV SLNITSLGLRSLKEISDGDVIISGN KNLCYANTINWKKLFGTSGQKTKII SNRGENSCKATGQVCHALCSPEGCW GPEPRDCVSCRNVSRGRECVDKCNL LEGEPREFVENSECIQCHPECLPQA MNITCTGRGPDNCIQCAHYIDGPHC VKTCPAGVMGENNTLVWKYADAGHV CHLCHPNCTYGCTGPGLEGCPTNGP KIPSGLNDIFEAQKIEWHEHHHHHH |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
Products Types
◆ Lysates | ||
EGFR-1621MCL | Recombinant Mouse EGFR cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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