Recombinant Human EHHADH Protein, GST-tagged

• Cat.No.: EHHADH-3135H
• Product Overview: Human EHHADH full-length ORF ( NP_001957.2, 1 a.a. - 723 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 105.9 kDa
• AA Sequence: MAEYTRLHNALALIRLRNPPVNAIS TTLLRDIKEGLQKAVIDHTIKAIVI CGAEGKFSAGADIRGFSAPRTFGLT LGHVVDEIQRNEKPVVAAIQGMAFG GGLELALGCHYRIAHAEAQVGLPEV TLGLLPGARGTQLLPRLTGVPAALD LITSGRRILADEALKLGILDKVVNS DPVEEAIRFAQRVSDQPLESRRLCN KPIQSLPNMDSIFSEALLKMRRQHP GCLAQEACVRAVQAAVQYPYEVGIK KEEELFLYLLQSGQARALQYAFFAE RKANKWSTPSGASWKTASARPVSSV GVVGLGTMGRGIVISFARARIPVIA VDSDKNQLATANKMITSVLEKEASK MQQSGHPWSGPKPRLTSSVKELGGV DLVIEAVFEEMSLKKQVFAELSAVC KPEAFLCTNTSALDVDEIASSTDRP HLVIGTHFFSPAHVMKLLEVIPSQY SSPTTIATVMNLSKKIKKIGVVVGN CFGFVGNRMLNPYYNQAYFLLEEGS KPEEVDQVLEEFGFKMGPFRVSDLA GLDVGWKSRKGQGLTGPTLLPGTPA RKRGNRRYCPIPDVLCELGRFGQKT GKGWYQYDKPLGRIHKPDPWLSKFL SRYRKTHHIEPRTISQDEILERCLY SLINEAFRILGEGIAASPEHIDVVY LHGYGWPRHKGGPMFYASTVGLPTV LEKLQKYYRQNPDIPQLEPSDYLKK LASQGNPPLKEWQSLAGSPSSKL
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: EHHADH enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase [ Homo sapiens ]
• Official Symbol: EHHADH
• Synonyms: EHHADH; enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase; ECHD, enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase; peroxisomal bifunctional enzyme; PBE; 3,2-trans-enoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase; L-3-hydroxyacyl-CoA dehydrogenase; L-bifunctional protein, peroxisomal; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; LBP; ECHD; LBFP; PBFE; L-PBE; MGC120586
• Gene ID: 1962
• mRNA Refseq: NM_001166415
• Protein Refseq: NP_001159887
• MIM: 607037
• UniProt ID: Q08426