Recombinant Human EIF4E, His-tagged

• Cat.No.: EIF4E-2775H
• Product Overview: Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 27.2 kDa(237aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-217 aa
• Description: EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEH YIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSN LM PG CD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LET LL CLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II (2020)
  RNA Polymerase II is involved in 18S and 25S ribosomal RNA transcription, in Candida albicans (2019)

Gene Information

• Gene Name: EIF4E eukaryotic translation initiation factor 4E [ Homo sapiens ]
• Synonyms: eukaryotic translation initiation factor 4E; CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1; eIF4E
• Gene ID: 1977
• mRNA Refseq: NM_001130678
• Protein Refseq: NP_001124150
• MIM: 133440
• UniProt ID: P06730
• Chromosome Location: 4q21-q25
• Pathway: Insulin signaling pathway; mTOR signaling pathway; Gene Expression; Metabolism of RNA; Metabolism of proteins; Signaling by Insulin receptor
• Function: translation initiation factor activity; protein binding; RNA cap binding

Q&As

Can the protein bind mRNA in vitro in the absence of ATP and other proteins?

EIF4E-2775H is a full-length protein that contains all binding sites; we highly recommend you try this protein.