Recombinant Human EIF4E, His-tagged

• Cat.No.: EIF4E-2775H
• Product Overview: Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 27.2 kDa(237aa).

Specification

• Cat. No.: EIF4E-2775H
• Description: EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
• Source: E.coli.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEH YIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSN LM PG CD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LET LL CLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  RNA Polymerase II is involved in 18S and 25S ribosomal RNA transcription, in Candida albicans (2019)
  Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II (2020)

Gene Information

• Gene Name: EIF4E eukaryotic translation initiation factor 4E [ Homo sapiens ]
• Synonyms: eukaryotic translation initiation factor 4E; CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1; eIF4E
• Gene ID: 1977
• mRNA Refseq: NM_001130678
• Protein Refseq: NP_001124150
• MIM: 133440
• UniProt ID: P06730
• Chromosome Location: 4q21-q25
• Pathway: Insulin signaling pathway; mTOR signaling pathway; Gene Expression; Metabolism of RNA; Metabolism of proteins; Signaling by Insulin receptor
• Function: translation initiation factor activity; protein binding; RNA cap binding

Reviews

02/20/2019

researchers highly recommend the EIF4E protein due to its excellent performance in ELISA and its suitability for protein electron microscopy structure analysis.

02/09/2017

Researchers can rely on the EIF4E protein's specificity and sensitivity to obtain accurate and reliable results.

07/10/2016

The EIF4E protein comes highly recommended for a variety of applications, particularly in ELISA and protein electron microscopy structure analysis.

Q&As

How does EIF4E protein expression differ in cancer cells compared to normal cells?

Cancer cells often exhibit elevated levels of EIF4E expression, leading to increased protein synthesis. This heightened activity is associated with tumor growth and aggressiveness.

Are there any targeted therapies or drugs designed to modulate EIF4E protein activity?

Yes, researchers are exploring targeted therapies that aim to inhibit EIF4E activity in cancer cells. These therapies hold promise for treating certain types of cancer.

How does EIF4E protein contribute to the development of drug resistance in cancer cells?

Elevated EIF4E activity in cancer cells can lead to increased synthesis of proteins involved in drug resistance mechanisms. This can reduce the effectiveness of chemotherapy and targeted therapies.

Can EIF4E protein levels be used as a diagnostic or prognostic marker for cancer?

Yes, elevated EIF4E expression has been observed in some cancer types and is being investigated as a potential diagnostic and prognostic marker. However, further research is needed to establish its clinical utility.

Apart from cancer, are there any other diseases where dysregulated EIF4E protein has been implicated?

Yes, dysregulated EIF4E expression has been associated with neurological disorders and viral infections. Researchers are studying its role in these diseases to explore potential therapeutic interventions.