Recombinant Human ELOB Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ELOB-5792H
• Product Overview: TCEB2 MS Standard C13 and N15-labeled recombinant protein (NP_996896) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
• Molecular Mass: 17.7 kDa
• AA Sequence: MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVHLHVHSQTMAKNRNTSWSQCPGLTACSTREPQDGPTQVHPRWGLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ELOB elongin B [ Homo sapiens (human) ]
• Official Symbol: ELOB
• Synonyms: ELOB; elongin B; SIII; TCEB2; elongin-B; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII p18; elongin 18 kDa subunit; elongin, 18-kD subunit; epididymis secretory sperm binding protein; transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); transcription elongation factor B polypeptide 2; transcription elongation factor B subunit 2
• Gene ID: 6923
• mRNA Refseq: NM_207013
• Protein Refseq: NP_996896
• MIM: 600787
• UniProt ID: Q15370