| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
1-434 aa (full length) |
| Description : |
Alpha-enolase (ENO1) is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. It functions as a homodimer and is one of three enolase isoenzymes in mammals (alpha, beta, gamma). Beyond glycolysis, ENO1 also acts as a structural lens protein (tau-crystallin), a plasminogen receptor, and a transcriptional repressor (MBP-1 isoform) of the c-myc promoter. It plays roles in growth regulation, hypoxia tolerance, allergic responses, and fibrinolysis. |
| Form : |
Liquid |
| Molecular Mass : |
49 kDa (444 aa including His-tag), calculated |
| AASequence : |
HHHHHHHHHHMSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK |
| Endotoxin : |
<1 EU/µg (LAL method) |
| Purity : |
>90% (by SDS-PAGE) |
| Applications : |
SDS-PAGE, Western Blot, Enzyme Activity |
| Storage : |
Store under sterile conditions at -20°C to -80°C; aliquot to avoid repeated freeze-thaw cycles |
| Concentration : |
0.46 mg/ml (BCA assay) |
| EC : |
4.2.1.11 |
| Storage Buffer : |
Sterile PBS, pH 7.4 |
