Recombinant Human ENO1 Protein ENO1-002H

Recombinant Human ENO1 Protein

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Recombinant Human ENO1 Protein

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Cat.No. : ENO1-002H
Product Overview : Recombinant full length human ENO1 protein (1-434) without tag was expressed in E. coli.
Description : This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Source : E. coli
Species : Human
Form : TBS, pH 7.4
Molecular Mass : 47.74 kDa
Protein length : 1-434
Purity : ≥90% estimated by SDS-PAGE
Stability : ≥ 1 year
Storage : Store at -80 centigrade
Shipping : Dry ice in continental US; may vary elsewhere
SDS-PAGE analysis of citrullinated α-enolase. :

Lane 1: MW Markers
Lane 2: 4 µg α-Enolase (PADI4 Citrullinated)
Lane 3: 2 µg α-Enolase (PADI4 Citrullinated)

Western blot analysis of α-enolase citrullination. :

α-Enolase and citrullinated α-enolase were reacted with Citrullinated α-Enolase Monoclonal Anybody (Clone 8D3) and detected using Goat And Mouse IgG HRP.
Lane 1: Citrullinated α-Enolase (20 ng)
Lane 2: Citrullinated α-Enolase (100 ng)
Lane 3: Citrullinated α-Enolase (400 ng)
Lane 4: α-Enolase (20 ng)
Lane 5: α-Enolase (100 ng)
Lane 6: α-Enolase (400 ng)

Gene Name : ENO1 enolase 1 [ Homo sapiens (human) ]
Official Symbol : ENO1
Synonyms : ENO1; enolase 1; NNE; PPH; MPB1; ENO1L1; HEL-S-17; alpha-enolase; c-myc promoter-binding protein-1; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; non-neural enolase; phosphopyruvate hydratase; plasminogen-binding protein; tau-crystallin; EC 4.2.1.11
Gene ID : 2023
mRNA Refseq : NM_001201483
Protein Refseq : NP_001188412
MIM : 172430
UniProt ID : P06733

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