Recombinant Human ENO1 Protein (Ser2-Lys434), N-His tagged, Animal-free, Carrier-free

• Cat.No.: ENO1-74H
• Product Overview: Recombinant Human ENO1 Protein (Ser2-Lys434) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Enolase 1 (ENO1) also named alpha-enolase which is a glycolytic enzyme. It could convert the NAD+ to NADH by producing two ATP. ENO1 is a homodimer which is composed by two isozymes of enolase (2 α, 2 ν, or 2 β). It also plays an important role in cancer model which can promote tumor cell proliferation and migration by the PI3K signaling pathway. ENO1 is a biomarker of prognostic and diagnostic cancer.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 47.85 kDa.
  The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ser2-Lys434
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS with 7 mM MgSO₄, pH 7.2
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: ENO1 enolase 1, (alpha) [ Homo sapiens (human) ]
• Official Symbol: ENO1
• Synonyms: ENO1; enolase 1, (alpha); ENO1L1, MPB1; alpha-enolase; c-myc promoter-binding protein-1; MBP 1; PPH; alpha-enolase; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase; NNE; MPB1; ENO1L1
• Gene ID: 2023
• mRNA Refseq: NM_001201483
• Protein Refseq: NP_001188412
• MIM: 172430
• UniProt ID: P06733