Recombinant Human ENPP2 Protein, His-tagged ENPP2-013H

Recombinant Human ENPP2 Protein, His-tagged

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Recombinant Human ENPP2 Protein, His-tagged

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Cat.No. : ENPP2-013H
Product Overview : Recombinant Human ENPP2 protein with a C-terminal histidine-tag was expressed in Sf21 cells
Description : The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.
Source : Insect Cells
Species : Human
Tag : His
Molecular Mass : 98.8 kDa
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 µmol of p-nitrophenol per minute at 37 centigrade in 50 mM Tris-HCl, pH 9.0, containing bis-nitrophenol phosphate.
Stability : ≥ 9 months
Storage : -80 centigrade
Storage Buffer : 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
Activity of ENPP2 :

SDS-PAGE analysis of ENPP2 :

Lane 1: MW Markers
Lane 2: Autotaxin (2 µg)
Lane 3: Autotaxin (4 µg)

Gene Name : ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 [ Homo sapiens (human) ]
Official Symbol : ENPP2
Synonyms : ENPP2; ectonucleotide pyrophosphatase/phosphodiesterase 2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D; EC 3.1.4.39
Gene ID : 5168
mRNA Refseq : NM_001040092
Protein Refseq : NP_001035181
MIM : 601060
UniProt ID : Q13822

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