Active Recombinant Human ENTPD1 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: ENTPD1-04H
• Product Overview: Biotinylated Human CD39, His,Avitag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1).

Specification

• Description: CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells(Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
• Bio-activity: Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 3,000 pmol/min/μg.
• Molecular Mass: The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Thr 38 - Val 478
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  The product MUST be stored at -70°C or lower upon receipt;
  -70°C for 3 months under sterile conditions.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ENTPD1 ectonucleoside triphosphate diphosphohydrolase 1 [ Homo sapiens ]
• Official Symbol: ENTPD1
• Synonyms: CD39;ENTPD1;NTPDase 1;Entpd1;Ecto-ATPDase 1;Ecto-ATPase 1
• Gene ID: 953
• mRNA Refseq: NM_001098175
• Protein Refseq: NP_001091645
• MIM: 601752
• UniProt ID: P49961