Recombinant Human EPCAM Protein, His & Avi-tagged, Biotinylated

• Cat.No.: EPCAM-350H
• Product Overview: Recombinant Human EPCAM Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). Predicted N-terminus: Gln 24. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 30 kDa. The protein migrates as 36-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: 30 kDa
• Protein length: Gln 24 - Lys 265
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: EPCAM
• Synonyms: EPCAM; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2
• Gene ID: 4072
• mRNA Refseq: NM_002354
• Protein Refseq: NP_002345
• MIM: 185535
• UniProt ID: P16422