Recombinant Human EpCAM protein(Met1-Lys265), His-Avi-tagged, Biotinylated

• Cat.No.: EPCAM-350H
• Product Overview: Biotinylated Recombinant Human EPCAM (NP_002345.2) (Met1-Lys265) was expressed in HEK293 with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Met1-Lys265
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind Human E-Cadherin Protein, Recombinant (ECD, hFc Tag) in functional ELISA.
• Molecular Mass: The recombinant human EPCAM consists of 268 amino acids and predicts a molecular mass of 30.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: EpCAM
• Synonyms: EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2
• Gene ID: 4072
• mRNA Refseq: NM_002354
• Protein Refseq: NP_002345
• MIM: 185535
• UniProt ID: P16422

Reviews

02/13/2022

Reliable for assays, satisfied customer.

09/09/2019

High-quality product, consistent results.

04/03/2019

Quick delivery, product as described.

Q&As

In which types of tissues is EpCAM predominantly expressed?

EpCAM is highly expressed in epithelial tissues and some cancer cells.

How does EpCAM contribute to cell adhesion?

It has a controversial role in cell adhesion, possibly contributing to both cell-cell adhesion and anti-adhesive properties.

What is the primary function of EpCAM in cells?

EpCAM primarily functions in cell signaling, proliferation, and migration.

What role does EpCAM play in cancer progression?

It often has a pro-tumorigenic role, promoting cancer cell proliferation, migration, and invasion.

How is EpCAM used as a biomarker in cancer diagnosis?

EpCAM is used as a biomarker to detect and isolate circulating tumor cells.

What therapeutic strategies are being explored involving EpCAM?

Strategies include developing EpCAM-targeted therapies for cancer treatment.

Are there known genetic alterations in EpCAM associated with specific health conditions?

Genetic alterations in EpCAM are linked to some hereditary cancer syndromes.