Recombinant Human EPHA1 Protein, GST-tagged

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Recombinant Human EPHA1 Protein, GST-tagged

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Cat.No. : EPHA1-3374H
Product Overview : Human EPHA1 (NP_005223.3, 586 a.a. - 976 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]
Source : Insect cells
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
Substrate: Blk/Lyntide.
ATP: 100 μM.
Molecular Mass : 72 kDa
Purity : 82 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : EPHA1 EPH receptor A1 [ Homo sapiens ]
Official Symbol : EPHA1
Synonyms : EPHA1; EPH receptor A1; EphA1 , EPHT, EPHT1; ephrin type-A receptor 1; EPH; hEpha1; oncogene EPH; eph tyrosine kinase 1; soluble EPHA1 variant 1; soluble EPHA1 variant 2; tyrosine-protein kinase receptor EPH; erythropoietin-producing hepatoma receptor; erythropoietin-producing hepatoma amplified sequence; EPHT; EPHT1; MGC163163;
Gene ID : 2041
mRNA Refseq : NM_005232
Protein Refseq : NP_005223
MIM : 179610
UniProt ID : P21709

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