Active Recombinant Human EPHB1 Protein, His-tagged

• Cat.No.: EPHB1-01H
• Product Overview: Recombinant human EPHB1 (18-540aa), fused to His-tag at Cterminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human EFNB1.
• Molecular Mass: 59.2 kDa (529aa)
• AA Sequence: MEETLMDTRTATAELGWTANPASGW EEVSGYDENLNTIRTYQVCNVFEPN QNNWLLTTFINRRGAHRIYTEMRFT VRDCSSLPNVPGSCKETFNLYYYET DSVIATKKSAFWSEAPYLKVDTIAA DESFSQVDFGGRLMKVNTEVRSFGP LTRNGFYLAFQDYGACMSLLSVRVF FKKCPSIVQNFAVFPETMTGAESTS LVIARGTCIPNAEEVDVPIKLYCNG DGEWMVPIGRCTCKPGYEPENSVAC KACPAGTFKASQEAEGCSHCPSNSR SPAEASPICTCRTGYYRADFDPPEV ACTSVPSGPRNVISIVNETSIILEW HPPRETGGRDDVTYNIICKKCRADR RSCSRCDDNVEFVPRQLGLTECRVS ISSLWAHTPYTFDIQAINGVSSKSP FPPQHVSVNITTNQAAPSTVPIMHQ VSATMRSITLSWPQPEQPNGIILDY EIRYYEKEHNEFNSSMARSQTNTAR IDGLRPGMVYVVQVRARTVAGYGKF SGKMCFQTLTDDDYKSELREQLP
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: EPHB1 EPH receptor B1 [ Homo sapiens (human) ]
• Official Symbol: EPHB1
• Synonyms: EPHB1; EPH receptor B1; ELK; NET; Hek6; EPHT2; ephrin type-B receptor 1; EK6; EPH-like kinase 6; eph tyrosine kinase 2; neuronally-expressed EPH-related tyrosine kinase; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; EC 2.7.10.1
• Gene ID: 2047
• mRNA Refseq: NM_004441
• Protein Refseq: NP_004432
• MIM: 600600
• UniProt ID: P54762