Recombinant Human EPHB2 protein, His-tagged

• Cat.No.: EPHB2-694H
• Product Overview: Recombinant Human EPHB2 protein(P29323-1)(Val19-Leu543), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Val19-Leu543
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EPHB2 EPH receptor B2 [ Homo sapiens ]
• Official Symbol: EPHB2
• Synonyms: EPHB2; EPH receptor B2; DRT, EphB2 , EPHT3, ERK; ephrin type-B receptor 2; Hek5; Tyro5; EPH-like kinase 5; eph tyrosine kinase 3; elk-related tyrosine kinase; protein-tyrosine kinase HEK5; tyrosine-protein kinase TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase receptor EPH-3; developmentally-regulated Eph-related tyrosine kinase; DRT; EK5; ERK; CAPB; PCBC; EPHT3; MGC87492
• Gene ID: 2048
• mRNA Refseq: NM_004442
• Protein Refseq: NP_004433
• MIM: 600997
• UniProt ID: P29323