Active Recombinant Human EPHB3 protein, His-tagged, Low Endotoxin

• Cat.No.: EPHB3-563H
• Product Overview: Recombinant Human EPHB3 protein(P54753)(Gly34-Leu559), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 34-559 aa
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EphB3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EPHB3 EPH receptor B3 [ Homo sapiens ]
• Official Symbol: EPHB3
• Synonyms: EPHB3; EPH receptor B3; EphB3, ETK2; ephrin type-B receptor 3; Hek2; Tyro6; EK2; EPH-like kinase 2; embryonic kinase 2; human embryo kinase 2; EPH-like tyrosine kinase 2; EPH-like tyrosine kinase-2; tyrosine-protein kinase TYRO6; ETK2; HEK2; TYRO6
• Gene ID: 2049
• mRNA Refseq: NM_004443
• Protein Refseq: NP_004434
• MIM: 601839
• UniProt ID: P54753