Recombinant Human EPHB4 Protein, GST-tagged

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Recombinant Human EPHB4 Protein, GST-tagged

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Cat.No. : EPHB4-3403H
Product Overview : Human EPHB4 (NP_004435.3, 577 a.a. - 987 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Description : Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]
Source : Insect cells
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate: Blk/Lyntide.
ATP: 100 μM.
Molecular Mass : 73 kDa
Purity : 82 % by SDS-PAGE/CBB staining.
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : EPHB4 EPH receptor B4 [ Homo sapiens ]
Official Symbol : EPHB4
Synonyms : EPHB4; EPH receptor B4; EphB4 , HTK; ephrin type-B receptor 4; Tyro11; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; HTK; MYK1; TYRO11;
Gene ID : 2050
mRNA Refseq : NM_004444
Protein Refseq : NP_004435
MIM : 600011
UniProt ID : P54760
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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