Recombinant Human Epidermal Growth Factor Receptor, Strep-tagged

• Cat.No.: EGFR-893H
• Product Overview: The EGFR contains the extracellular domain of the human EGFR (25-647 a.a.) excluding the signal peptide which is cleaved by the insect cells having an approximate Mw of 80kDa. The EGFR is fused to a C-terminal Strep-tag and purified by proprietary chromatographic techniques.

Specification

• Cat. No.: EGFR-893H
• Description: The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4).
• Source: Human
• Host: Sf9 Insect Cells
• Form: ErbB1 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1x PBS pH=7.4.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized EGFR in sterile PBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: LEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCK LLEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPSIA + Strep-tag
• Storage: Lyophilized EGFR although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGFR should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; ERBB; ERBB1; mENA; HER1; PIG61; Receptor tyrosine-protein kinase erB-1; Proto-oncogene c-ErbB-1; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); OTTHUMP00000209210; cell growth inhibiting protein 40; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); cell proliferation-inducing protein 61; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; erythroblastic leukemia viral (v-erb-b) oncogene homolog; EC. 2.7.10.1
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: Q9GZX1
• Chromosome Location: 7p12
• Function: ATP binding; MAP/ERK kinase kinase activity; actin filament binding; double-stranded DNA binding; epidermal growth factor receptor activity; identical protein binding; contributes to nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transferase activity; transmembrane receptor activity