Recombinant Human ERBB2 protein, His-Avi-tagged
Cat.No. : | ERBB2-083H |
Product Overview : | Human ErbB2/Her2 Protein is expressed from mammalian with a His and Avi tag at the C-terminal. It contains Thr 23 - Thr 652. |
- Specification
- Gene Information
- Related Products
Description : | ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. |
Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Form : | Supplied as 0.22um filtered solution in PBS (pH 7.4). |
Molecular Mass : | The Protein has a calculated MW of 72.3kDa .As a result of glycosylation, the protein migrates as 80-100kDa based on Bis-Tris PAGE. |
Protein length : | It contains Thr 23 - Thr 652. |
Endotoxin : | Less than 0.05EU per ug by the LAL method. |
Purity : | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Storage : | The product should be stored at -70°C. Please do not repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ] |
Official Symbol : | ERBB2 |
Synonyms : | D340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian) |
Gene ID : | 2064 |
mRNA Refseq : | NM_001005862 |
Protein Refseq : | NP_001005862 |
MIM : | 164870 |
UniProt ID : | P04626 |
Products Types
◆ Recombinant Protein | ||
Erbb2-969M | Recombinant Mouse Erbb2 Protein, MYC/DDK-tagged | +Inquiry |
ERBB2-6H | Recombinant Human ERBB2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ERBB2-360H | Active Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated | +Inquiry |
ERBB2-3441H | Active Recombinant Human ERBB2 Protein | +Inquiry |
ERBB2-3444H | Recombinant Human ERBB2 Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
ERBB2-465HCL | Recombinant Human ERBB2 cell lysate | +Inquiry |
ERBB2-2008RCL | Recombinant Rhesus ERBB2 cell lysate | +Inquiry |
ERBB2-2658HCL | Recombinant Human ERBB2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (8)
Ask a question1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.
ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.
Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.
ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.
ERBB2 overexpression is often associated with aggressive disease and poor prognosis.
ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.
It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.
Amplification of the ERBB2 gene leads to its overexpression in cancer cells.
Customer Reviews (3)
Write a reviewWorked as expected, satisfied.
Reliable in protein assays.
Quick and reliable shipping.
Ask a Question for All ERBB2 Products
Required fields are marked with *
My Review for All ERBB2 Products
Required fields are marked with *
Inquiry Basket