Active Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated

• Cat.No.: ERBB2-360H
• Product Overview: Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1). Predicted N-terminus: Thr 23. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-95 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.2-6 ng/mL (QC tested).
• Molecular Mass: 73.0 kDa
• Protein length: Thr 23 - Thr 652
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; NGL; TKR1; HER-2; MLN 19; HER-2/neu
• Gene ID: 2064
• mRNA Refseq: NM_001005862
• Protein Refseq: NP_001005862
• MIM: 164870
• UniProt ID: P04626