Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated

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Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated

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Cat.No. : ERBB2-360H
Product Overview : Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1). Predicted N-terminus: Thr 23. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-95 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : His & Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human ERBB2 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.2-6 ng/mL (QC tested).
Molecular Mass : 73.0 kDa
Protein length : Thr 23 - Thr 652
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
Official Symbol : ERBB2
Synonyms : ERBB2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; NGL; TKR1; HER-2; MLN 19; HER-2/neu;
Gene ID : 2064
mRNA Refseq : NM_001005862
Protein Refseq : NP_001005862
MIM : 164870
UniProt ID : P04626

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