Active Recombinant Human ERBB4 protein, hFc&His-tagged

• Cat.No.: ERBB4-69H
• Product Overview: Recombinant Human ERBB4 protein(Gln26-Arg649), fused with C-terminal hFc and His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 26-649 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human NRG1-beta1 at 2 μg/mL (100 μL/well) can bind Recombinant Human HER4/ErbB4, the EC50 of HER4/ErbB4 is 20.99 ng/mL. 2. Measured by its binding ability in a functional ELISA.Immobilized Human ErbB4/Her4 Antibody at 1μg/mL (25 μL/well) can bind Human ErbB4/Her4 with a linear range of 0.46-14.6 ng/mL.
• Molecular Mass: 96.43 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: ERBB4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) [ Homo sapiens ]
• Official Symbol: ERBB4
• Synonyms: ERBB4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v erb a avian erythroblastic leukemia viral oncogene homolog like 4; receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; HER4; p180erbB4; MGC138404
• Gene ID: 2066
• mRNA Refseq: NM_001042599
• Protein Refseq: NP_001036064
• MIM: 600543
• UniProt ID: Q15303