Recombinant Human ERCC3 Protein, GST-tagged

• Cat.No.: ERCC3-3459H
• Product Overview: Human ERCC3 full-length ORF ( NP_000113.1, 1 a.a. - 782 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 115.7 kDa
• AA Sequence: MGKRDRADRDKKKSRKRHYEDEEDD EEDAPGNDPQEAVPSAAGKQVDESG TKVDEYGAKDYRLQMPLKDDHTSRP LWVAPDGHIFLEAFSPVYKYAQDFL VAIAEPVCRPTHVHEYKLTAYSLYA AVSVGLQTSDITEYLRKLSKTGVPD GIMQFIKLCTVSYGKVKLVLKHNRY FVESCHPDVIQHLLQDPVIRECRLR NSEGEATELITETFTSKSAISKTAE SSGGPSTSRVTDPQGKSDIPMDLFD FYEQMDKDEEEEEETQTVSFEVKQE MIEELQKRCIHLEYPLLAEYDFRND SVNPDINIDLKPTAVLRPYQEKSLR KMFGNGRARSGVIVLPCGAGKSLVG VTAACTVRKRCLVLGNSAVSVEQWK AQFKMWSTIDDSQICRFTSDAKDKP IGCSVAISTYSMLGHTTKRSWEAER VMEWLKTQEWGLMILDEVHTIPAKM FRRVLTIVQAHCKLGLTATLVREDD KIVDLNFLIGPKLYEANWMELQNNG YIAKVQCAEVWCPMSPEFYREYVAI KTKKRILLYTMNPNKFRACQFLIKF HERRNDKIIVFADNVFALKEYAIRL NKPYIYGPTSQGERMQILQNFKHNP KINTIFISKVGDTSFDLPEANVLIQ ISSHGGSRRQEAQRLGRVLRAKKGM VAEEYNAFFYSLVSQDTQEMAYSTK RQRFLVDQGYSFKVITKLAGMEEED LAFSTKEEQQQLLQKVLAATDLDAE EEVVAGEFGSRSSQASRRFGTMSSM SGADDTVYMEYHSSRSKAPSKHVHP LFKRFRK
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ERCC3 excision repair cross-complementing rodent repair deficiency, complementation group 3 [ Homo sapiens ]
• Official Symbol: ERCC3
• Synonyms: ERCC3; excision repair cross-complementing rodent repair deficiency, complementation group 3; TFIIH basal transcription factor complex helicase XPB subunit; BTF2; GTF2H; RAD25; TFIIH; xeroderma pigmentosum group B complementing; XPB; BTF2 p89; TFIIH p89; TFIIH 89 kDa subunit; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; basic transcription factor 2 89 kDa subunit; xeroderma pigmentosum, complementation group B; xeroderma pigmentosum group B-complementing protein; TFIIH basal transcription factor complex 89 kDa subunit
• Gene ID: 2071
• mRNA Refseq: NM_000122
• Protein Refseq: NP_000113
• MIM: 133510
• UniProt ID: P19447