| Cat.No. : |
ESPL1-3502H |
| Product Overview : |
Human ESPL1 partial ORF ( NP_036423, 586 a.a. - 701 a.a.) recombinant protein with GST-tag at N-terminal. |
| Description : |
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010] |
| Source : |
Wheat Germ |
| Species : |
Human |
| Tag : |
GST |
| Molecular Mass : |
38.5 kDa |
| AA Sequence : |
REELQAYKAV RADTGQERFN IICDLLELSP EETPAGAWAR ATHLVELAQV LCYHDFTQQT NCSALDAIRE ALQLLDSVRP EAQARDQLLD DKAQALLWLY ICTLEAKIQE GIERDR |
| Applications : |
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array |
| Notes : |
Best use within three months from the date of receipt of this protein. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Quality Control Testing : |
12.5% SDS-PAGE Stained with Coomassie Blue.
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