Recombinant Human ESPL1 Protein, GST-tagged

• Cat.No.: ESPL1-3502H
• Product Overview: Human ESPL1 partial ORF ( NP_036423, 586 a.a. - 701 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 38.5 kDa
• AA Sequence: REELQAYKAVRADTGQERFNIICDL LELSPEETPAGAWARATHLVELAQV LCYHDFTQQTNCSALDAIREALQLL DSVRPEAQARDQLLDDKAQALLWLY ICTLEAKIQEGIERDR
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ESPL1 extra spindle pole bodies homolog 1 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ESPL1
• Synonyms: ESPL1; extra spindle pole bodies homolog 1 (S. cerevisiae); extra spindle poles like 1 (S. cerevisiae); separin; ESP1; KIAA0165; SEPA; separase; caspase-like protein ESPL1; extra spindle poles like 1; extra spindle poles-like 1 protein; FLJ46492
• Gene ID: 9700
• mRNA Refseq: NM_012291
• Protein Refseq: NP_036423
• MIM: 604143
• UniProt ID: Q14674