Recombinant Human ESPL1 Protein, GST-tagged

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Recombinant Human ESPL1 Protein, GST-tagged

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Cat.No. : ESPL1-3502H
Product Overview : Human ESPL1 partial ORF ( NP_036423, 586 a.a. - 701 a.a.) recombinant protein with GST-tag at N-terminal.
Description : Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 38.5 kDa
AA Sequence : REELQAYKAV RADTGQERFN IICDLLELSP EETPAGAWAR ATHLVELAQV LCYHDFTQQT NCSALDAIRE ALQLLDSVRP EAQARDQLLD DKAQALLWLY ICTLEAKIQE GIERDR
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : ESPL1 extra spindle pole bodies homolog 1 (S. cerevisiae) [ Homo sapiens ]
Official Symbol : ESPL1
Synonyms : ESPL1; extra spindle pole bodies homolog 1 (S. cerevisiae); extra spindle poles like 1 (S. cerevisiae); separin; ESP1; KIAA0165; SEPA; separase; caspase-like protein ESPL1; extra spindle poles like 1; extra spindle poles-like 1 protein; FLJ46492;
Gene ID : 9700
mRNA Refseq : NM_012291
Protein Refseq : NP_036423
MIM : 604143
UniProt ID : Q14674

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